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High gradient magnetic cell separation with MACS

A flexible fast and simple magnetic cell sorting system for separation of large numbers of cells according to specific cell surface markers was developed and tested. Cells stained sequentially with biotinylated antibodies fluorochrome‐conjugated avidin and superparamagnetic biotinylated‐microparticles (about 100 nm diameter) are

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Magnetic Separatoran overview ScienceDirect Topics

Barry A. Wills James A. Finch FRSC FCIM P.Eng. in Wills Mineral Processing Technology (Eighth Edition) 2016 13.4.3 Material Transport in Magnetic Separators. Commercial magnetic separators are continuous-process machines and separation is carried out on a moving stream of particles passing into and through the magnetic field. Close control of the speed of passage of the particles

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Considerations for high-yield high-throughput cell

 · Fong C. Y. Peh G. S. Gauthaman K. Bongso A. Separation of SSEA-4 and TRA-1-60 labelled undifferentiated human embryonic stem cells from a heterogeneous cell population using magnetic

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MagAttract Magnetic Rack Handbook

The MagAttract Magnetic Rack is a convenient separation device for nucleic acid purification using magnetic beads (e.g. MagAttract Suspension G) in 2 ml tubes. The beads are pulled to the side of the 2 ml tube by a magnetic field which has a strength of >50 megaoersted and are held in place while solutions are exchanged or removed.

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Magnetic RacksGenScript

GenScript AmMag™ MR-mini magnetic separation rack is designed to separate GenScript s paramagnetic beads from solution contained in 1.5 mL and 2 mL micro-centrifuge tubes. The magnetic field has been optimized for separation of tubes . 2 mL in volume

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EriezResources

Laboratory Services. Offering sample testing with flotation magnetic and hydraulic separation and filtration equipment Eriez Central Test Lab (CTL) has a highly qualified staff with years of expertise along with a broad range of application experience.

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MagniSort Magnetic Cell Separation Technology Thermo

Use magnetic cell separation for less noise in your final results. Invitrogen MagniSort technology is designed to offer column-free magnetic separation platform for cell enrichment that is simpler faster and offers significant cost-savings compared to column-based separation methods. When absolute purity is not necessary as is often the case

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EriezBar Magnets

The Eriez Magna-Bar packs more magnetic strength in its tiny construction than any available magnet of comparable size and type. Installation is quick and simple. Removable equally spaced angle mounting brackets are furnished for ease of installation and to permit adjusting of the mounted magnets. Magna-Bars are ideal for use as a powerful yet

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An Introduction to Gas Chromatography Mass

 · Theory of column efficiency in chromatography Band broadening in chromatography is the result of several factors which influence the efficiency of separations. The separation efficiency of a column can be expressed in terms of the number of theoretical plates in the column. H = Hthe plate height (has dimensions of length µm)

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Magbeads 101 A guide to choosing and using magnetic

 · Magnetic separation uses a magnetic field to separate micrometer-sized paramagnetic particles from a suspension. In molecular biology magnetic beads provide a simple and reliable method of purifying various types of biomolecule including genomic DNA plasmids mitochondrial DNA RNA and proteins.

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Magnetic bead for protein and antibody purification

GenScript has been continuously developing excellent protein antibody magnetic beads products to meet researchers needs in proteomics research and other fields. A comprehensive line of GenScrit MagBeads are available for you to achieve rapid and efficient biomagnetic separation and ensure reliable and reproducible results.

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Magnets for Molecular and Cell Separation Applications

Description. DynaMag-2. 10 µL–2 000 µL. (This magnet replaces the Dynal MPC-S magnet) Holds 16 (1.5–2 mL) microfuge tubes. 8 positions on each side of the rack. Top rack can be quickly removed from the magnet in the base ready for vortexing rotation or manual sample shaking. A center pin in the rack ensures uniform vortexing of all tubes.

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Integrated DNA and RNA extraction using magnetic beads

 · Current extraction methods often extract DNA and RNA separately and few methods are capable of co-extracting DNA and RNA from sputum. We established a nucleic acid co-extraction method from

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Microfluidic sorting of intrinsically magnetic cells under

 · In distinction to column-based magnetic sorting devices microfluidic systems can prevent sample contact with superparamagnetic material. This ensures contamination-free separation of naturally

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50 mL Tube Magnetic SeparatorThermo Fisher Scientific

This Magnetic Separator is for use with the GeneCatcher gDNA 310 ml Blood Kit. The Magnetic Separator consists of a rack that holds 4 50-ml tubes in alignment with neodymium magnets for the efficient processing of DNA bound to magnetic beads. GeneCatcher Technology is a novel magnetic bead-based

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Gas‐assisted low‐field magnetic separation for large scale

 · In this study the gas-assisted low-field magnetic separation (GAMS) was proposed for continuous large-scale magnetic bio-separation process. Magnetic recovery processes in selective separation of binary mixed proteins by magnetic microspheres were selected as a model.

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Cell Isolation Products and Cell Separation Technologies

Isolate cells in as little as 8 minutes with our widely-used cell separation systems. EasySep™ RosetteSep™ and SepMate™ are column-free cell separation technologies with fast and easy protocols. RoboSep™ instruments allow for fully automated cell isolation based on the EasySep™ column

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Bio-On-Magnetic-Beads (BOMB) Open platform for high

 · The protocol we have developed (S1 Appendix BOMB protocol 9.1) follows a fast optimised bisulfite conversion chemistry however for the separation of converted DNA and following desulfonation steps silica-coated BOMB beads are employed instead of column-based purification thus allowing treatment of many samples in parallel.

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Functional analysis of novel Rab Wiley Online Library

For immuno-magnetic separation the LCVs in macrophage lysates were treated with an antibody against SidC followed by a secondary antibody coupled to magnetic beads. The LCVs were passed over a MACS (magnetic cell separation) column washed and eluted by removing the column form the magnet. After this proce-

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How to Isolate Cells with EasySep™ Column-Free Cell

 · EasySep™ is a fast easy and column-free magnetic cell separation technology that allows you to isolate highly purified cells in as little as 8 minutes. This video will take you through the isolation of human CD4 T cells by negative selection using EasySep™. Publish Date May 03 2016 Views 19075.

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ProtocolsProImmuneMastering Immunity _ MHC

Protocols for Cell Separation using Magnetic Beads. Guide to Cell Separation using Magnetic Beads. Column-based bead isolation for fluorescent Pentamers. Column-based bead isolation for biotin-labeled Pentamers. Tube-based bead isolation for biotin-labeled Pentamers. CD137 isolation and staining protocol . Cell Preparation Protocols

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50 mL Tube Magnetic SeparatorThermo Fisher Scientific

This Magnetic Separator is for use with the GeneCatcher gDNA 310 ml Blood Kit. The Magnetic Separator consists of a rack that holds 4 50-ml tubes in alignment with neodymium magnets for the efficient processing of DNA bound to magnetic beads. GeneCatcher Technology is a novel magnetic

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MagniSort Magnetic Cell Separation Technology Thermo

Use magnetic cell separation for less noise in your final results. Invitrogen MagniSort technology is designed to offer column-free magnetic separation platform for cell enrichment that is simpler faster and offers significant cost-savings compared to column-based separation methods.

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Electromagnetic SeperatorsWorld Authority in Separation

Suspended Electromagnets. The Suspended Electromagnet providing tramp metal collection from conveyed materials is a widely used magnetic separator. The electromagnet is typically mounted or suspended over a conveyor belt to remove large pieces of tramp metal that represent a hazard to downstream crushers mills pulverizers and grinders.

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Cell separation columns Magnetic cell separation

MACS® Columns for magnetic cell separation with minimal labeling. MACS® Column-based cell separations are fast and gentle to any cell type flowing through. When using our MACS® MicroBeads along with MACS® Columns you can rely on excellent yields and purity of cells with minimal labeling. Cell functionality is preserved following separation

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Preparation of Concanavalin A-Chelating Magnetic

 · In this work a soft and nondestructive approach was developed to prepare concanavalin A-chelating magnetic nanoparticles (Con A-MNPs) for selective enrichment of glycoproteins. Ethylenediamine tetraacetic acid-modified-MNPs (EDTA-MNPs) were prepared by a one-pot chemical coprecipitation method first and then Cu(II) cations were used as bridge groups to immobilize Con A

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BioMagnetic Research and Technology BioMed Central

 · Magnetic separation technology using magnetic part icles is quick and easy method for sensitive and reliable capture of specific proteins genetic ma terial and other biomolec ules. The technique offers an advantage in terms of subjecting the analyte to very little mechanical stress compared to other methods.

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Electromagnetic SeperatorsWorld Authority in

Suspended Electromagnets. The Suspended Electromagnet providing tramp metal collection from conveyed materials is a widely used magnetic separator. The electromagnet is typically mounted or suspended over a conveyor belt to remove large pieces of tramp metal that represent a hazard to downstream crushers mills pulverizers and grinders.

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The History of Dynabeads and Biomagnetic Separation

The History of Dynabeads and Biomagnetic Separation. Dynal is built on a major breakthrough that revolutionized the separation of biological materials. In 1976 the Norwegian professor John Ugelstad first succeeded in making spherical polystyrene beads of exactly the same size—only previously achieved by NASA in the weightless conditions of

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DNA Reference GuideThermo Fisher Scientific

 · reagents all the way to spin columns and magnetic beads. Table 1. Summary of DNA purification technologies. Method Organic extraction Column Magnetic beads Chemistry Separation via phenolic compounds Separation via filtration membranes or resins seated at the bottom of a column tube Separation via magnetic beads that act as charged surfaces to

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Preparations of magnetic molecularly imprinted polymer

 · Chromatographic separation was performed with a Sino-Chrom ODS-AP column (5 µm 230 mm 4.6 mm) (Dalian Elite Analytical Instruments Co. Ltd Dalian China). The mobile phase was consisted of methanol and KH 2 PO 4 (0.05 mol l −1 ) in a ratio

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Cell Separation Cell Isolation Products Miltenyi

MACS® Cell SeparationSelect the best technology for your cell isolation We offer cell isolation methods tailored to your target cell needs. Our unique cell separation portfolio combines our proven magnetic cell isolation technology with exciting new options providing for workflows across basic and clinical research.

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Magnetic reconnection at the Wiley Online Library

 · Magnetic reconnection is known to be an important process for coupling solar wind mass and momentum into the Earth s magnetosphere. Reconnection is initiated in an electron-scale dissipation/diffusion region around an X line but its consequences are large scale.

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Integrated DNA and RNA extraction using magnetic beads

 · Current extraction methods often extract DNA and RNA separately and few methods are capable of co-extracting DNA and RNA from sputum. We established a

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96-Well Microtiter Plate Magnetic Separation Rack 96

The 96-well Microtiter Plate Magnetic Separation Rack is designed to be used with commercially available high-flanged 100 µl to 300 µl flat-bottom 96-well microplates. 24 side-pull magnetic pins attract magnetic beads from solution to the side walls of four adjacent wells. The orientation of the magnetic field ensures complete removal of the

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Messenger RNABIA Separations

Messenger RNA is large for its mass. It is also sensitive to shear forces. The large channel structure of the chromatographic monolith allows free access for large particles and laminar flow prevents shear damage. The structure enables purification of mRNA up to 10 kb or larger. The rich spectrum of chemical properties of mRNA is the basis for

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